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目的:观察CBX7对宫颈癌细胞增殖和侵袭的影响,并探讨其可能的机制。方法:收集本院50例宫颈癌患者行手术切除后的宫颈肿瘤组织及癌旁组织,检测CBX7mRNA和蛋白的表达情况。采用siRNA技术下调Hela细胞中CBX7的表达,通过MTT和侵袭实验检测细胞的增殖和侵袭能力,Western blot检测内质网应激相关蛋白(GRP78、PERK、ATF6、IRE1)的表达情况。在CBX7下调后加入内质网应激诱导剂(Tuniamycin),检测肿瘤细胞的增殖情况。结果:宫颈肿瘤组织中CBX7的蛋白及mRNA水平显著高于癌旁组织(均P<0.05)。CBX7下调后,Hela细胞的增殖和侵袭能力较对照组显著降低(均P <0.05),内质网应激相关蛋白(GRP78、PERK、ATF6、IRE1)的表达明显下降,而内质网应激诱导剂(Tuniamycin)可促进细胞增殖能力恢复。结论:CBX7可通过调控内质网应激促进宫颈癌细胞的增殖和侵袭。
Abstract：Objective:To observe the effect of CBX7 on proliferation and invasion of cervical cancer cells,and to explore its possible mechanism.Methods:The mRNA level and protein expression of CBX7 in tumor tissues and adjacent tissues of 50 patients with cervical cancer were detected.On the basis of siRNA interference technology,the proliferation and invasion of Hela cells were evaluated under the condition of CBX7down-regulation.Endoplasmic reticulum stress(ERS)related proteins,including GRP78,PERK,ATF6 and IRE1were all detected by Western blot.ESR inducer(Tuniamycin)was used to test restoration of cell proliferation after down-regulation of CBX7.Results:The mRNA level and protein expression of CBX7 in tumor tissues was significantly higher than that in adjacent tissues(both P<0.05).Down-regulation of CBX7 significantly reduced proliferation and invasion of Hela cells(both P <0.05),and decreased ESR related proteins GRP78,PERK,ATF6 and IRE1when compared to the control group.In addition,the inhibitory effect of CBX7down-regulation on Hela cells proliferation was eliminated by ESR inducer(Tuniamycin).Conclusion:CBX7can promote the proliferation and invasion of cervical cancer cells through regulation of ESR.
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基本信息:
中图分类号:R737.33
引用信息:
[1]罗幼珍,王清,张胜初.CBX7通过促进内质网应激增强宫颈癌细胞的增殖和侵袭[J].巴楚医学,2018,1(04):46-50.
2018-12-30
2018-12-30